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Streptococcus salivarius K12ÀÇ ¹è¾çÁ¶°Ç¿¡ µû¸¥ Ç×±ÕÈ°¼ºÀÇ Æ¯Â¡

Characteristics of antimicrobial activity of Streptococcus salivarius K12 by culture condition

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¼Û¿µ±Õ ( Song Young-Gyun ) - Dankook University College of Dentistry Department of Prosthodontics
À̼ºÈÆ ( Lee Sung-Hoon ) - Dankook University College of Dentistry Department of Oral Microbiology and Immunology

Abstract

¸ñÀû: º» ¿¬±¸ÀÇ ¸ñÀûÀº Streptococcus salivarius K12ÀÇ ¼ºÀå°ú Ç×±ÕÈ°¼º¿¡ ´ëÇÑ ¹è¾çÁ¶°ÇÀÇ ¿µÇâÀ» ¾Ë¾Æº¸´Â °ÍÀÌ´Ù.

¿¬±¸ Àç·á ¹× ¹æ¹ý: S. salivarius K12´Â µ¿¹° ¶Ç´Â ½Ä¹° ´Ü¹éÁúÀ» ÇÔÀ¯ÇÑ ¹èÁö ¶Ç´Â Áß¼º ¹× »ê¼º Á¶°ÇÀÇ ¹èÁö¿¡¼­ ¹è¾çµÇ¾ú´Ù. S. salivarius K12ÀÇ ¼ºÀåÀº 2½Ã°£¸¶´Ù ºÐ±¤±¤µµ°è·Î ÃøÁ¤ÇÏ¿´´Ù. S. salivarius K12ÀÇ Streptococcus mutans, Porphyromonas gingivalis ¹× Candida albicans¿¡ ´ëÇÑ Ç×±Õ ¶Ç´Â Ç×Áø±Õ È°¼ºÀ» ¹è¾ç¾×À» ÀÌ¿ëÇÑ °¨¼ö¼º ºÐ¼®À¸·Î Á¶»çÇÏ¿´´Ù.

°á°ú: S. salivarius K12ÀÇ ¼ºÀåÀº ½Ä¹° ´Ü¹éÁú°ú Áß¼º pH Á¶°Ç¿¡¼­ ´õ ºü¸¥ ¼ºÀåÀ» º¸¿´´Ù. S. salivarius K12ÀÇ Ç×±Õ ¹× Ç×Áø±Õ È°¼ºÀº µ¿¹°¼º ´Ü¹éÁúº¸´Ù ½Ä¹°¼º ´Ü¹éÁúÀ» ÇÔÀ¯ÇÑ ¹èÁö¿¡¼­ ´õ °­ÇÏ°Ô ³ªÅ¸³µ´Ù.

°á·Ð: S. salivarius K12¸¦ ¼¼±Õ¼º ±¸°­Áúȯ¿¡ Àû¿ëÇϱâ À§Çؼ­´Â S. salivarius K12°¡ ±¸°­ ³»¿¡¼­ ±ºÁýÈ­ÇÏ¿© Ç×±Õ È°¼ºÀ» Çâ»ó½ÃÅ°±â À§ÇÑ º¸Á¶¹°ÁúÀÌ ÇÊ¿äÇÒ ¼ö ÀÖ´Ù.

Purpose: The purpose of this study was to examine effects of culture conditions on the growth and antibacterial activity of Streptococcus salivarius K12.

Materials and Methods: S. salivarius K12 was cultivated in medium containing animal and plant protein or in medium of neutral and acidic conditions. The growth of S. salivarius K12 was measured every 2 hours by a spectrophotometer. The antimicrobial activity of S. salivarius K12 against Streptococcus mutans and Porphyromonas gingivalis was investigated by the susceptibility assay using the spent culture medium.

Results: the growth of S. salivarius K12 showed faster in medium containing plant protein and neutral pH condition. The antimicrobial and antifungal activity of S. salivarius K12 appeared stronger in medium containing plant protein than animal proteins.

Conclusion: For application of S. salivarius K12 to bacterial oral disease, co-substances may be needed for S. salivarius K12 to colonize in the oral cavity and enhance the antimicrobial activity.

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S. salivarius K12; ÇÁ·Î¹ÙÀÌ¿Àƽ½º; ÇÁ¸®¹ÙÀÌ¿Àƽ½º; Ç×±Õ È°¼º
S. salivarius K12; probiotics; prebiotics; antimicrobial activity

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